[ Papers ]

Journal of the Korean Society of Manufacturing Technology Engineers - Vol. 31, No. 2, pp.116-122

ISSN: 2508-5107 (Online)

Print publication date 15 Apr 2022

Received 24 Feb 2022 Revised 01 Apr 2022 Accepted 06 Apr 2022

DOI: https://doi.org/10.7735/ksmte.2022.31.2.116

유액흐름 분석을 통한 바이오 폴리머 생산 효모의 교반조건 최적화

강경곤^a^{, b} ; 김두현^a^{, c} ; 권덕호^a^{, b} ; 장웅기^a^{, d} ; 김병희^a^{, d} ; 하호진^a^{, c} ; 하석진^a^{, b}^{, *}

Optimization of Agitation Conditions for Biopolymer Production using Yeast through Fluid Flow Analysis

Kyoung-Gon Kang^a^{, b} ; Doohyeon Kim^a^{, c} ; Deok-Ho Kwon^a^{, b} ; Woong Ki Jang^a^{, d} ; Byeon Hee Kim^a^{, d} ; Hojin Ha^a^{, c} ; Suk-Jin Ha^a^{, b}^{, *}

aDepartment of Biohealth-machinery Convergence Engineering, Kangwon National University
bDepartment of Bioengineering and Technology, Kangwon National University
cDepartment of Mechanical and Biomedical Engineering, Kangwon National University
dDepartment of Mechanical and Mechatronics Engineering, Kangwon National University

Correspondence to: ^*Tel.: +82-33-250-6275 E-mail address: sjha@kangwon.ac.kr (Suk-Jin Ha).

Abstract

The medium used for microbial fermentation is generally in a liquid state, that is, a fluid. Here, there is no fixed shape, and the flow is irregular. Attempts have been made to analyze and predict the flow of the fluid. Kluyveromyces marxianus JMA-1 was developed to produce 3-hydroxy propionic acid (3-HP), a biopolymer precursor, and the agitation conditions were optimized by applying hydrodynamics. The maximum flow velocity and Γ1 value indicating the degree of rotation were the highest (45.35 cm/s and 0.72, respectively) in the control (Baffle 0) and the lowest (20.99 cm/s and 0.40, respectively) in Baffle 2. However, 3-HP production was the highest (8.82 ± 0.24 g/L) in Baffle 2 and the lowest (6.83 ± 0.11 g/L) in the control. The 3-HP production of Baffle 2 improved by 29% compared to that of the control.

Keywords:

3-hydroxy propionic acid (3-HP), Fluid engineering, Bio-engineering, Fluid flow

Acknowledgments

이 논문은 2020년도 정부(교육부)의 재원으로 한국연구재단의 4단계 BK21 사업의 지원을 받아 수행된 연구 결과임.

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Kyoung-Gon Kang

M.S. degree in Department of Bioengineering and Technology, Kangwon National University. His research interest is metabolic and genetic engineering.

E-mail: 202016278@kangwon.ac.kr

Doohyeon Kim

M.S. degree in Department of Mechanical and Biomedical Engineering, Kangwon National University. His research interest is fluid mechanics and particle image velocimetry and computational fluid dynamics.

E-mail: kdhyeon96@kangwon.ac.kr

Deok-Ho Kwon

Research professor in the department of Biohealth-machinery convergence engineering, Kangwon National University. His research interest is metabolic and genetic engineering.

E-mail: deok-ho@kangwon.ac.kr

Woong Ki Jang

Research professor in the department of Biohealth-machinery convergence engineering, Kangwon National University. His research interest is MEMS based surgace texturing technologies and mechanical design.

E-mail: wkddndrl@kangwon.ac.kr

Byeon Hee Kim

Professor in the Department of Mechanical and Mechatronics Engineering, Kangwon National University. His research interest is the design of medical devices and AI application system design.

E-mail: kdh@kangwon.ac.kr

Hojin Ha

Professor in the Department of Mechanical and Biomedical Engineering, Kangwon National University. His research interest is fluid mechanics and particle image velocimetry and computational fluid dynamics and 4D flow MRI.

E-mail: hojinha@kangwon.ac.kr

Suk-Jin Ha

Professor in the Department of Bioengineering and Technology, Kangwon National University. His research interest is production of bio-fuel and bio-plastic using microorganism.

E-mail: sjha@kangwon.ac.kr