[ Papers ]

Journal of the Korean Society of Manufacturing Technology Engineers - Vol. 28, No. 5, pp.286-292

ISSN: 2508-5107 (Online)

Print publication date 15 Oct 2019

Received 06 Sep 2019 Revised 10 Oct 2019 Accepted 14 Oct 2019

DOI: https://doi.org/10.7735/ksmte.2019.28.5.286

바이오의약품 정제용 크로마토그래피 컬럼의 고효율 흐름 분배기 개발

고영석^a^{, c}

; 이화정^b ; 최수림^b ; 김동일^c ; 박진호^a^{, *}

; 이준철^a

Development of the Highly Efficient Flow Distributor of the Chromatography Column for Biopharmaceutical Purification

Young-Seok Ko^a^{, c}

; HwaJung Ri^b ; Su-lim Choi^b ; Dong-Il Kim^c ; Jean-Ho Park^a^{, *}

; Joon-Chul Lee^a

aKorea Institute of Industrial Technology, 143, Hanggaul-ro, Sangrok-gu, Ansan, Gyeonggi-do, 15588, Korea
bAmicogen, 700, Daewangpangyo-ro, Bundang-gu, Seongnam, Gyeonggi-do, 13488, Korea
cDepartment of Biological Engineering, Inha university, 100, Inharo, Nam-gu, Incheon 22212, Korea

Correspondence to: ^*Tel.: +82-31-8084-8822 Fax: +82-31-8084-8830 E-mail address: Jeanho@kitech.re.kr (Jean-Ho Park).

Abstract

We developed a flow distributor for high-performance chromatographic processes during the purification of biopharmaceuticals. Twelve distributor candidates are provided, including four types of distributors with different-shaped holes, and three sub-types with different number of holes. We evaluated the flow distributor candidates by spreading bromophenol blue dye on the membrane , and selected the most suitable distributor Arc 45 deg. type. Using 10 ml columns with three different length-to-diamter (L/D) ratios (4.33, 0.92, and 0.34) of column, we measured peak asymmetry (As) values. The 10 ml column with 0.92 L/D ratio showed best As value. We also carried out flow experiments on the three sub-types of Arc 45 deg. distributors on the 0.92 L/D ratio 50 ml column and Arc 45 deg. 1:8 distributor showed best performance. The As values of this optimal flow distributor are 1.2 or less. In summary, this study provides optimal distributor values for chromatographic purification of biopharmaceuticals.

Keywords:

Flow distributor, Chromatography, Column, 3D print, Biopharmaceutical purification

Acknowledgments

이 연구는 중소벤처기업부 산학연협력 기술개발사업의 지원으로 수행 되었으며, 이에 관계자 여러분께 감사드립니다(SE180029).

References

Stankovich, J., 2013, The Impact of Column Connection on Band Broadening in Very High Pressure Liquid Chromatography, Journal of Seperation Science, 36:17 2709-2717. [https://doi.org/10.1002/jssc.201300175]
Fekete, S., 2011, The Impact of Extra-Column Band Broadening on the Chromatographic Efficiency of 5 cm Narrow-Bore Very Efficient Columns, Journal of Chromatography A, 1218:31 5286-5291. [https://doi.org/10.1016/j.chroma.2011.06.045]
Smits, W., 2014, The Impact of Flow Distribution on Column Performance: A Computational Fluid Dynamics Study, Journal of Chromatography A. 1369:21 125-130. [https://doi.org/10.1016/j.chroma.2014.10.024]
Farkas, T., 1996, Column Radial Homogeneity in High-Performance Liquid Chromatography, Journal of Chromatography A. 740:2 169-181. [https://doi.org/10.1016/0021-9673(96)00107-0]
Farkas, T., 1997, Radial Distribution of the Flow Velocity, Efficiency and Concentration in a Wide HPLC Column, American Institute of Chemical Engineers Journals. 43:8 1964-1974. [https://doi.org/10.1002/aic.690430806]
Farkas, T., 1997, Contribution of the Radial Distribution of the Flow Velocity to Band Broadening in HPLC Columns, Analytical Chemistry. 69:22 4592-4600. [https://doi.org/10.1021/ac970530m]
Tallarek, U., 1995, NMR Imaging of the Chromatographic Process Migration and Separtation of Bands of Gadolinium Chelates, Journal of Chromatography A. 696:1 1-18. [https://doi.org/10.1016/0021-9673(94)01231-3]
Tallarek, U., 1996, Measurement of Transverse and Axial Apparent Dispersion Coefficients in Packed Beds, American Institute of Chemical Engineers Journals. 42:11 3041-3054. [https://doi.org/10.1002/aic.690421106]
Shalliker, A., 1999, Visualization of Sample Introduction in Liquid Chromatographic Columns Contribution of a Flow Distributor on the Sample Band Shape, Journal of Chromatography A. 865:1-2 83-95. [https://doi.org/10.1016/S0021-9673(99)00852-3]
Pizzo, S. M., Moraes, D., 1998, Analysis of Liquid Distribution in a Packed Column on a Pilot Scale, Industrial & Engineering Chemistry Research. 37:7 2844-2849. [https://doi.org/10.1021/ie970785q]
Leva, M., 1953, Tower Packings and Packed Tower Design, United States Stoneware Co., Akron.
Treybal, R. E., 1980, Mass-Transfer Operations, McGraw-Hill Inc., New York.
Kister, H. Z., 1992, Distillation Design, McGraw-Hill Inc., New York.
Bemer, G. G., Zuiderweg, F. J., 1978, Radial Liquid Spread and Maldistribution in Packed Columns under Different Wetting Conditions, Chemical Engineering Science. 33:12 1637 - 1643. [https://doi.org/10.1016/0009-2509(78)85140-9]
Engelhardt, H., 2012, Practice of High Performance Liquid Chromatography: Applications, Equipment, and Quantitative Analysis, Springer Science & Business Media, Berlin, Heidelberg
Busnel, J.P., 2001, Investigation and Interpretation of Band Broadening in Size Exclusion Chromatography, Journal of Chromatography A, 930:1-2 61-71. [https://doi.org/10.1016/S0021-9673(01)01159-1]
MicroSolv Technology Corporation, 23 Jul.2016, viewed 12 september 2019, Knowledge Base Home: Asymmetry Factor, http://kb.mtc-usa.com/article/AA-01463/0/Asymmetry-factor.html, .
GE Life Sciences, n.d., viewed 18 October 2019, XK Column Packing, http://www.gelifesciences.co.kr/wp-content/uploads/2017/01/XK-column-packing_narration.pdf, .