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Journal of the Korean Society of Manufacturing Technology Engineers - Vol. 28 , No. 5

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Journal of the Korean Society of Manufacturing Technology Engineers - Vol. 28, No. 5, pp. 286-292
Abbreviation: J. Korean Soc. Manuf. Technol. Eng.
ISSN: 2508-5107 (Online)
Print publication date 15 Oct 2019
Received 06 Sep 2019 Revised 10 Oct 2019 Accepted 14 Oct 2019
DOI: https://doi.org/10.7735/ksmte.2019.28.5.286
바이오의약품 정제용 크로마토그래피 컬럼의 고효율 흐름 분배기 개발
고영석^a^{, c} ; 이화정^b ; 최수림^b ; 김동일^c ; 박진호^a^{, *} ; 이준철^a



Development of the Highly Efficient Flow Distributor of the Chromatography Column for Biopharmaceutical Purification
Young-Seok Ko^a^{, c} ; HwaJung Ri^b ; Su-lim Choi^b ; Dong-Il Kim^c ; Jean-Ho Park^a^{, *} ; Joon-Chul Lee^a
aKorea Institute of Industrial Technology, 143, Hanggaul-ro, Sangrok-gu, Ansan, Gyeonggi-do, 15588, Korea
bAmicogen, 700, Daewangpangyo-ro, Bundang-gu, Seongnam, Gyeonggi-do, 13488, Korea
cDepartment of Biological Engineering, Inha university, 100, Inharo, Nam-gu, Incheon 22212, Korea
Correspondence to : ^*Tel.: +82-31-8084-8822 Fax: +82-31-8084-8830 E-mail address: Jeanho@kitech.re.kr (Jean-Ho Park).



Funding Information ▼ Ministry of SMEs and Startups SE180029

Abstract

We developed a flow distributor for high-performance chromatographic processes during the purification of biopharmaceuticals. Twelve distributor candidates are provided, including four types of distributors with different-shaped holes, and three sub-types with different number of holes. We evaluated the flow distributor candidates by spreading bromophenol blue dye on the membrane , and selected the most suitable distributor Arc 45 deg. type. Using 10 ml columns with three different length-to-diamter (L/D) ratios (4.33, 0.92, and 0.34) of column, we measured peak asymmetry (As) values. The 10 ml column with 0.92 L/D ratio showed best As value. We also carried out flow experiments on the three sub-types of Arc 45 deg. distributors on the 0.92 L/D ratio 50 ml column and Arc 45 deg. 1:8 distributor showed best performance. The As values of this optimal flow distributor are 1.2 or less. In summary, this study provides optimal distributor values for chromatographic purification of biopharmaceuticals.


Keywords: Flow distributor, Chromatography, Column, 3D print, Biopharmaceutical purification

Acknowledgments

이 연구는 중소벤처기업부 산학연협력 기술개발사업의 지원으로 수행 되었으며, 이에 관계자 여러분께 감사드립니다(SE180029).

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